transwell inserts  (Servicebio Inc)

Journal: Bioactive Materials

Article Title: Reconstructing the ischemic osteogenic microenvironment through hierarchical scaffolds orchestrating Mg 2+ signaling and neuropilin-1–mediated angiogenesis

doi: 10.1016/j.bioactmat.2026.02.031

Figure Lengend Snippet: Evaluation of angiogenic potential induced by Mg 2+ and NRP-1. ( A ) Scratch assay in three different microenvironments. ( B ) Transwell assay in three different microenvironments. ( C ) Quantitative analysis of wound healing area. ( D ) Quantitative analysis of number of migration cells. ( E ) Immunofluorescence staining of VEGFA and FGF2, with DAPI for nuclear staining and F-actin for cytoskeleton labeling. ( F ) Tube formation evaluation in four different microenvironments. ( G ) Quantitative analysis of number of junction. ( H ) Quantitative analysis of Flu intensity. ( I ) Western-blot analysis of VEGFA, FGF2, and Nr4a1. ( J-L ) Quantitative analysis of relative protein expression of VEGFA, FGF2, and Nr4a1. Data are presented as mean values ± s.d. (n = 3). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 (one-way ANOVA).

Article Snippet: For the Transwell assay, BMSCs were seeded in the upper chambers of Transwell inserts (8.0 μm pore size; Servicebio, China), while different culture conditions were applied in the lower chambers according to the experimental groups.

Techniques: Wound Healing Assay, Transwell Assay, Migration, Immunofluorescence, Staining, Labeling, Western Blot, Expressing

Journal: Frontiers in Immunology

Article Title: Multi-omics profiling identifies ADAM9 as a key efferocytosis driver in lung adenocarcinoma

doi: 10.3389/fimmu.2026.1772167

Figure Lengend Snippet: Effects of ADAM9 knockdown in macrophage cells. (A) Relative mRNA expression of ADAM9 in LUAD tissues and adjacent tissues. (B) ADAM9 expression in LUAD tissues evaluated by IHC. (C, D) ADAM9 expression in M2 macrophages transfected with siRNA. (E) RT-qPCR analysis of M2 polarization markers in si-ADAM9-transfected M2 macrophages. (F) ATP content assay in M2 macrophages transfected with si-ADAM9. (G) ADAM9 mRNA expression in normal bronchial epithelial cells and LUAD cell lines. (H) Transwell migration assay showing the migratory ability of A549 and H1975 cells co-cultured with M2 macrophages. (I, J) EdU assay detecting the proliferative capacity of LUAD cells co-cultured with M2 macrophages. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: Cells were pre-cultured in serum-free medium for 48 h. Then, 2.0×10 4 cells were seeded in the upper chamber of a transwell insert (NEST Biotechnology, Wuxi, China), while the lower chamber was filled with RPMI-1640 medium containing 10% FBS (Bioland, China).

Techniques: Knockdown, Expressing, Transfection, Quantitative RT-PCR, Transwell Migration Assay, Cell Culture, EdU Assay

Journal: Translational Cancer Research

Article Title: Molecular mechanism of miR-27a-3p targeting FBXW7 regulating the malignant behavior of osteosarcoma cells

doi: 10.21037/tcr-2025-aw-2290

Figure Lengend Snippet: The role of miR-27a-3p in osteosarcoma cells. (A) Expression levels of miR-27a-3p in normal osteoblasts hFOB1.19 and osteosarcoma cells Saos-2 and MG-63. (B,C) The influence of introducing miR-27a-3p mimic and inhibitor on the expression of miR-27a-3p in osteosarcoma cell lines MG-63 and SAOS-2. (D-F) CCK-8 assay, Transwell migration assay and FCM assay were used to detect the changes in cell proliferation, migration ability and apoptosis levels of osteosarcoma cells MG-63 and Saos-2 transfected with miR-27a-3p mimic and inhibitor. Stained with crystal violet; magnification: ×200. *, P<0.05; **, P<0.01. CCK-8, Cell Counting Kit-8; FCM, flow cytometry; NC, negative control.

Article Snippet: A volume of 100 μL of this cell suspension was carefully introduced into the upper chamber of the Transwell insert (Servicebio, Cat. No. C6610), and 600 μL of medium supplemented with 10% fetal bovine serum was added to each well of the lower chamber.

Techniques: Expressing, CCK-8 Assay, Transwell Migration Assay, Migration, Transfection, Staining, Cell Counting, Flow Cytometry, Negative Control

Journal: Translational Cancer Research

Article Title: Molecular mechanism of miR-27a-3p targeting FBXW7 regulating the malignant behavior of osteosarcoma cells

doi: 10.21037/tcr-2025-aw-2290

Figure Lengend Snippet: The targeted regulation of miR-27a-3p on FBXW7. (A,B) Effect of miR-27a-3p inhibitor combined with siFBXW7 on the expression of FBXW7 in osteosarcoma cells MG-63 (A) and Saos-2 (B). (C,D) CCK-8 assay (C), Transwell migration assay (D) were used to detect the changes in cell proliferation and migration ability levels of osteosarcoma cells MG63 and Saos-2 transfected with miR-27a-3p inhibitor+siFBXW7. Stained with crystal violet; magnification: ×200. *, P<0.05; **, P<0.01; ***, P<0.001. CCK-8, Cell Counting Kit-8; NC, negative control.

Article Snippet: A volume of 100 μL of this cell suspension was carefully introduced into the upper chamber of the Transwell insert (Servicebio, Cat. No. C6610), and 600 μL of medium supplemented with 10% fetal bovine serum was added to each well of the lower chamber.

Techniques: Expressing, CCK-8 Assay, Transwell Migration Assay, Migration, Transfection, Staining, Cell Counting, Negative Control

Journal: Translational Cancer Research

Article Title: Molecular mechanism of miR-27a-3p targeting FBXW7 regulating the malignant behavior of osteosarcoma cells

doi: 10.21037/tcr-2025-aw-2290

Figure Lengend Snippet: The role of FBXW7 in osteosarcoma cells. (A,B) CCK-8 assay, Transwell migration assay were used to detect the changes in cell proliferation and migration ability of osteosarcoma cells MG-63 and Saos-2 transfected with pcDNA3.1-FLAG-FBXW7 and siRNA-FBXW7. Stained with crystal violet; magnification: ×200. *, P<0.05; **, P<0.01. CCK-8, Cell Counting Kit-8; NC, negative control; OE, overexpression.

Article Snippet: A volume of 100 μL of this cell suspension was carefully introduced into the upper chamber of the Transwell insert (Servicebio, Cat. No. C6610), and 600 μL of medium supplemented with 10% fetal bovine serum was added to each well of the lower chamber.

Techniques: CCK-8 Assay, Transwell Migration Assay, Migration, Transfection, Staining, Cell Counting, Negative Control, Over Expression